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KMID : 1134820170460091061
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Journal of the Korean Society of Food Science and Nutrition
2017 Volume.46 No. 9 p.1061 ~ p.1070
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Physicochemical Properties and Protective Effects of Corni fructus Treated with Pressurized-Steam against H2O2-Induced Cytotoxicity on L132 Cells
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Park Hye-Mi
Hong Joo-Heon
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Abstract
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This study examined the physicochemical properties and protective effects of Corni fructus treated with pressurized-steam (through 121°C, 1.2 kgf/cm2, 0.5 h, 1 h, 2 h, and 3 h) against H2O2-induced cytotoxicity on L132 cells. The color values of the untreated Corni fructus powder were higher than those of Corni fructus after the pressurized-steam treatment (PSC), and those of PSC improved with a decrease in treatment time. At the observation by pressurized-steam treatment for more than 2 h, the color was changed to black, and its gloss was lost. The major constituents in PSC (2 hours) were the total sugar (468.53 mg/g), reducing sugar (385.55 mg/g), and total phenol (37.32 mg/g), respectively. The main components in the free sugars of PSC (2 h) were fructose, glucose, and sucrose, at 207.72 mg/g, 219.40 mg/g, and 4.31 mg/g, respectively. The gallic acid in the phenol compounds and 5-(hydroxymethyl) furfural in the furan compounds of PSC (2 h) improved with increasing treatment time. The main components in iridoid glycoside of PSC (2 h) were morroniside, loganin, and lognic acid, which improved with decreasing treatment time. The L132 cell growth inhibition activities of all the extracts were significantly higher than that of the control. The protective effects against the H2O2-induced cytotoxicity on L132 cells of PSC (2 h) was 102.82% (at 1,000 μg/mL) higher than those of the other extracts. This suggests that Corni fructus by PSC is useful for functional food materials in the food industry.
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KEYWORD
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Corni fructus, 5-(hydroxymethyl)furfural, iridoid glycoside, physicochemical property, protective effect
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